Ghrelin在驯鹿器官内的表达及定位检测

doi:10.11843/j.issn.0366-6964.2016.12.022

Abstract

Abstract:

The Real-time PCR and Immunohistochemistry were used to examine the expression and localization of Ghrelin in the reindeer organs. The results of Real-time PCR indicated that Ghrelin was expressed in 17 kinds of organs and the expression in the abomasum was highest, followed by pancreas, duodenal, testis and oesophagus, in which the expression of Ghrelin were remarkably higher than that of the rest organs (P<0.05). Immunohistochemical results showed that Ghrelin-positive cells in the oesophagus and abomasum mainly distributed in the tunica mucosa, tunica submucosa and tunica muscularis and also detected in the tunica mucosa, tunica submucosa of rumen, reticulum, omasum. In the intestine, Ghrelin-positive cells were mainly localized in the tunica mucosa, tunica submucosa and tunica muscularis of duodenum, jejumum, ileum, colon, especially more cells were found in the epithelium of intestinal villi and the tunica submucosa. Ghrelin-positive cells were observed in the liver, pancreas, lung, spleen, kidney, testis, anterior pituitary, thyroid gland. Real-time PCR and immunohistochemistry results showed that Ghrelin was presented in all of the organs that were examined, where the expression and distribution of Ghrelin in the esophagus and gastrointestinal were most widely, suggesting its possible role in regulating the digestive function.

CLC Number:

S852.1

ZHANG Man, JIN Xin, LIU Jiao, YANG Yin-feng. The Examination of Expression and Localization of Ghrelin in the Reindeer Organs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(12): 2510-2519.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2016.12.022

https://www.xmsyxb.com/EN/Y2016/V47/I12/2510

References

［1］KOJIMA M, HOSODA H, DATE Y, et al. Ghrelin is a growth-hormone-releasing acylated peptide from stomach［J］. Nature, 1999, 402 (6762): 656-660.
［2］ARIYASU H, TAKAYA K, TAGAMI T, et al. Stomach is a major source of ciculating ghrelin, and feeding state determines plasma ghrelin-like immunoreactivity levels in humans［J］. J Clin Endocrinol Metab, 2001, 86(10): 4753-4758.
［3］GNANAPAVAN S, KOLA B, BUSTIN S A, et al. The tissue distribution of the mRNA of ghrelin and subtypes of its receptor, GHS-R, in humans［J］. J Clin Endocrinol Metab, 2002, 87(6): 2988.
［4］李福宝, 方富贵, 李梅清, 等. Ghrelin在成年皖西白鹅小肠内的免疫组化定位研究［J］. 畜牧兽医学报, 2007, 38(2): 206-208.
LI F B, FANG F G, LI M Q, et al. Immunohistochemical localization of Ghrelin in the small intestine of adult Wanxi white goose［J］. Acta Veterinaria et Zootechica Sinica, 2007, 38(2): 206-208. (in Chinese)
［5］MILLER D W, HARRISON J L, BROWN Y A, et al. Immuohistochemical evidence for an endocrine/paracrine role for ghrelin in the reproductive tissues of sheep［J］. Reprod Biol Endocrinol, 2005, 3: 60.
［6］杨银凤, 余兴邦, 王冠玉, 等. 中国驯鹿生长素(Ghrelin)全长cDNA的克隆及序列分析［J］. 中国兽医学报,2014, 34(10): 1647-1652.
YANG Y F, YU X B, WANG G Y, et al. Cloning and analysis of the full-length cDNA of China reindeer Ghrelin［J］. Chinese Journal of Veterinary Science, 2014, 34(10): 1647-1652. (in Chinese)
［7］SHAO Y J, LIU S Q, TANG X Y, et al. Ontogeny of ghrelin mRNA expression and identification of ghrelin-immunopositive cells in the gastrointestinal tract of the Peking duck, Anas platyrhychos［J］. Gen Comp Endocrinol, 2010, 166(1): 12-18.
［8］KAIYA H, MIYAZATO M, KANGAWA K, et al. Ghrelin: a multifunctional hormone in non-mammalian vertebrates［J］. Comp Biochem Physiol A Mol Integr Physiol, 2008, 149(2): 109-128.
［9］曹贵方, 吕东媛, 白春玲, 等. 一种新的脑肠肽激素--Ghrelin［J］. 畜牧与饲料科学, 2004(6): 64-66.
CAO G F, LV D Y, BAI C L, et al. A new brain-gut peptide hormone-Ghrelin［J］. Animal Husbandry and Feed Science, 2004(6): 64-66. (in Chinese)
［10］WANG W, CHENG L, GUO J, et al. Expression of Ghrelin in gastrointestinal tract and the effect of early weaning on Ghrelin expression in lambs［J］. Mol Biol Rep, 2014, 41(2): 909-914.
［11］LIN T, MENG Q, SUI D, et al. Molecular cloning and expression analysis of porcine ghrelin O-acyltransferase［J］. Biochem Genet, 2011, 49（9-10）: 576-586.
［12］蒋书东, 戴书俊, 方富贵, 等. 黑麂肝、小肠和大肠的组织学结构及 Ghrelin 的分布［J］. 动物学杂志, 2012, 47(1): 36-43.
JIANG S D, DAI S J, FANG F G, et al. Histological structure and distribution of Ghrelin in liver，small intestine and large intestine of Muntiacus crinifrons［J］. Chinese Journal of Zoology, 2012, 47(1): 36-43. (in Chinese)
［13］魏凤梅, 李玉谷, 叶远兰, 等. Ghrelin免疫反应阳性细胞在鸡外周器官中的定位分布与发育性变化［J］. 畜牧兽医学报, 2010, 41(3): 341-346.
WEI F M, LI Y G, YE Y L, et al. Localization and development of Ghrelin-immunopositive cells in periphery organs of broiler chickens［J］. Acta Veterinaria et Zootechica Sinica, 2010, 41(3): 341-346. (in Chinese)
［14］张德敏. 济宁青山羊胃肠道Ghrelin免疫阳性细胞与小肠组织结构的发育性变化［D］. 泰安: 山东农业大学, 2012.
ZHANG D M. Developmental changes of Ghrelin-immunopositive cells in the gastrointestinal tract and morphological structure of small intestine in Jining gray goat［D］. Tai’an: Shandong Agricultural College, 2012. (in Chinese)
［15］SAKATA I, NAKAMURA K, YAMAZAKI M, et al. Ghrelin-producing cells exist as two types of cells, closed- and opened-type cells, in the rat gastrointestinal tract［J］. Peptides, 2002, 23(3): 531-536.
［16］NIEMINEN P, MUSTONEN A M, HYVÄRINEN H. Fasting reduces plasma leptin- and ghrelin-immunoreactive peptide concentrations of the burbot (Lota lota) at 2℃ but not at 10℃ ［J］. Zoolog Sci, 2003, 20(9) : 1109-1115.
［17］KAGEYAMA H, FUNAHASHI H, HIRAYAMA M, et al. Morphological analysis of ghrelin and its receptor distribution in the rat pancreas［J］. Regul Pept, 2005, 126(1-2): 67-71.
［18］TOLLE V, ZIZZARI P, TOMASETTO C, et al. In vivo and in vitro effects of Ghrelin/motilin-related peptide on growth hormone secretion in the rat ［J］. Neuroendocrinology, 2001, 73(1): 54-61.
［19］GAYTAN F, BARREIRO M L, CAMINOS J E, et al. Expression of ghrelin and its functional receptor, the type 1a growth hormone secretagogue receptor, in normal human testis and testicular tumors［J］. J Clin Endocrinol Metab, 2004, 89(1):400-409.

[1]	ZHU Jiaqiao, CHENG Laiyang, CAO Jiangqin, ZHU Min, LI Junwei, JU Huimin, LIU Zongping. Preliminary Study on the Location and Function of XRCC1 in Oocyte and Early Embryos [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2126-2133.
[2]	FANG Yingdong, CHENG Cuicui, LU Jia, CHENG Yujiao, ZHANG Rui, LI Peixuan, WANG Wenhui. Distribution Characteristics of Plasma Cells Secreting IgA and IgG in Rabbit Nasal Cavity [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2751-2762.
[3]	ZHANG Qian, CUI Yan, YU Sijiu, HE Junfeng, PAN Yangyang, WANG Meng. Distribution of Immune Cells in the Epididymis and Vas Deferens of Yaks (Bos grunniens) [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1925-1933.
[4]	HAN Yawen, LAI Zhenlin, ZHANG Mengmei, ZHAO Huiying, LIU Tengfei. Effect of 4℃ Liquid Storage on Ultrastructure of Dairy Goat Spermatozoa [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(3): 964-971.
[5]	ZHANG Yiling, KAN Zifei, NIU Zheng, YU Qiuhan, RAN Ling, ZHANG Shujuan, ZOU Hong, XU Shasha, ZHANG Jingyi, SONG Zhenhui. Preparation and Application of Precision-Cut Tissue Slices [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 339-348.
[6]	ZHANG Qian, CUI Yan, YU Sijiu, LIAO Bo, ZOU Shengnan, BAI Xuefeng, Seth Yaw Afedo, ZHAO Pengfei, LONG Min. Histological of Hair Follicles from Different Parts of Skin and Expression of TGF-β2 and HIF-1α in Young Yak (Bos grunniens) [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3224-3233.
[7]	ZHANG Zhidong, RYAN Eoin, LI Yanmin. Quantitative Analysis of FMDV Receptor Integrin mRNA Levels in Ovine Tissues at Different Developmental Stages [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2626-2632.
[8]	REN Yuxin, CUI Yan, ZOU Shengnan, BAI Xuefeng, YU Sijiu, HE Junfeng, LIAO Bo. Histological Observation of Blood Vessels and Nerves and Expression of HIF-1α in Adult Yaks Skin [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2633-2641.
[9]	LI Yuejiao, CUI Yan, ZHANG Qian, HE Junfeng. Study on the Distribution and Expression of CTGF and FGF-2 in the Lungs of Yaks at Different Ages [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(7): 2025-2033.
[10]	CHEN Jiaqi, ZHANG Xiaodi, GU Zhaobing, Lü Longbao, WU Dongwang, WANG Rongjiao, MAO Huaming. Effects and Mechanism of Faecal Microbiota Transplantation on Intestinal Barrier Function in Piglets [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(5): 1195-1207.
[11]	ZHANG Chen, FENG Luqiu, LIN Hailan, HUANG Ziqing, WANG Naixiu, GAN Ling. Association Analysis of Differentially Expressed miRNAs and Transcriptomics in Piglet Hippocampus under Oxidative Stress [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(5): 1396-1406.
[12]	DU Xiaohua, MI Xiaoyu, WANG Haifang, WEN Yongqiang, LI Qiao, LIU Xia. Expression and Localization of Neuroglobin and Hypoxia Inducible Factor-1α in Yak's Hindbrain [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(3): 789-798.
[13]	KONG Fuli, LIU Ranran, ZHAO Guiping, WEN Jie. Research Progress on Wooden Breast and White Striping Myopathies in Broilers [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(10): 2329-2340.
[14]	WANG Yali, CUI Yan, WANG Ji. Analysis of Casts of the Kidney Collection System Anatomical Structure in the Yak [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(10): 2584-2589.
[15]	BAI Xuefeng, CUI Yan, YU Sijiu, LI Hui, HE Junfeng, LIAO Bo. Effect of HIF-1α on Renal Tubular Epithelial-to-Mesenchymal Transition in Yak [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 620-629.

The Examination of Expression and Localization of Ghrelin in the Reindeer Organs

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments